Genomics Core Facility

Overview of Services

The Genomics Core Facility is housed within the Lewis-Sigler Institute for Integrative Genomics at Princeton University. The facility provides researchers with access to state of the art, high-throughput sequencing (both short reads on Illumina and long reads on PacBio platforms) and spatial transcriptomics technologies. In addition, the core facility offers sequencing library preparation services, consultation on experimental design and data analysis. Collaborations on developing novel sequencing applications are also welcome. The staff provide training on use of the self service equipment, troubleshooting, and assistance with software and data analysis. The Core Facility works closely with the Research Computing Staff to provide researchers with computing power and consulting services to analyze the sequencing and spatial transcriptomics data.

The primary contact for the Genomics Core Facility is Wei Wang, the Director, ww3@princeton.edu, 609-258-6998.

Disclaimer of Data Security

LSI computing and research data storage services are not intended or appropriate for human subjects, or sensitive data or data covered by data use agreements that require specific security or privacy controls.

Please contact LSI computing support (csgenome@princeton.edu) for assistance or guidance on resources that may be appropriate for this or other restricted data.

List of Services

Full Service

• Illumina Sequencing: NovaSeq 6000 and Miseq i100 Plus
• Illumina Library Prep: DNA, ATAC-seq, various RNA-seq
• PacBio Sequencing: Revio HiFi long reads using SPRQ chemistry
• PacBio Library Prep: DNA, optional Ampli-Fi, RNA by Iso-seq and Kinnex kits
• Single-cell sequencing prep: Chromium X from 10x Genomics
• Spatial in situ  transcriptomics: Xenium from 10x Genomics
• DNA and RNA QC by Bioanalyzer

Self Service

• ddPCR on BioRad QX200 with AutoDG
• QPCR on LifeTech Viia 7
• Agilent TapeStation
• NanoDrop One and NanoDrop Flex
• Biotek H1 plate reader
• Covaris ME220 sonicator
• BluePippin DNA size selection
• Kingfisher Flex robot for DNA / RNA extraction
• Revvity Janus G3 Liquid Handling Robot

Getting Started

• Princeton Clients: If you already have an account, log in (upper right corner) and go to the Request Services or Schedule Equipment tabs.  If you are a new user, log in (upper right corner) using a valid Princeton ID.
• Non-Princeton Clients: If you already have an account, log in (upper right corner) with approved iLab credentials.  If you are a new external user, Register to setup an iLab account.
• Visit the iLab Help Site or Customer Help Manual for further guidance using iLab's Core Facility Services.
• Sample storage: if you would like to take back your libraries or samples, please do so within 2 weeks after receiving the sequencing or other service results, otherwise your samples will be discarded afterwards.

Location and hours of operation

Links and Resources

1. http://lsi.princeton.edu/facilities/sequencing-facility
2. https://htseq.princeton.edu/

Acknowledgement

We appreciate that you have chosen our facility to conduct your study and would like to remind you to remember us when writing your manuscript, as authorship and acknowledgment are indicators of our facilities success.  If we've contributed to the experimental design, produced experimental data, and/or helped with analysis, interpretation or writing of the text then you should acknowledge us. If the work carried out has been exceptional or the person doing it has done significantly more than would normally be expected you might consider naming that person in the acknowledgment or adding that individual as an author on the paper. 

Examples:

"We thank the Princeton University Genomics Core Facility Staff for library preparation and sequencing."

Or

"We thank (individual staff member) and the Princeton University Genomics Core Facility for library preparation and sequencing."

Contacts