The Flow Cytometry Resource Facility provides support for faculty and student research at Princeton University, as well as for investigators outside of the university at other academic institutions or private companies. In addition to providing access to the flow cytometers and cell sorting services, we provide technical support to assist in experimental design, data analysis/interpretation and presentation as well as in the development of novel flow cytometric and FACS based techniques.
Flow cytometry is a laser based analytical tool in which cells or small organisms in suspension flow at high speed past one or more focused laser light sources. Fluorescently labeled probes and dyes can be added to the sample to determine specific physical and/or biochemical properties of the cells. Optical filters and detectors direct and measure the scattered laser light as well as the fluorescence that is emitted from the cell. Various software platforms are used to acquire and analyze the data, which can be exported in several formats for presentation.
The facility analyzes and sorts a wide range of cell types and species including human, murine, porcine and insect cell lines as well as primary murine leukocytes and stem cells, bacteria, yeast, C. elegans, and aquatic organisms, which reflects the diversity of the research at Princeton University.
The Flow Cytometry Resource Facility provides flow cytometry (FC) and fluorescence activated cell sorting (FACS) services, training for independent use of certain instrumentation, and expert consultation to assist with experimental design and troubleshooting, as well as data analysis/interpretation and presentation.
To request training, consultation, cell sorting and/or assisted services, please register with iLab.
Getting Started with iLab:
- Princeton Clients: If you already have an account, log in (upper right corner) and go to the Request Services or Schedule Equipment tabs. If you are a new user, log in (upper right corner) using a valid Princeton ID.
- Non-Princeton Clients: Please email firstname.lastname@example.org directly with any questions about use of this facility. If you already have an account, log in (upper right corner) with approved iLab credentials. If you are a new external user, Register to setup an iLab account.
- Visit the iLab Help Site or Customer Help Manual for further guidance using iLab's Core Facility Services.
The Flow Cytometry Resource Facility is available 24/7 for use of the flow cytometry benchtop analyzer and self-service FACS sorters by fully trained and authorized clients.
Staff assisted services are available Monday - Friday from 10am - 6pm, with after hours sort experiments available by special request.
Commonly Used Applications and Fluorochromes
We appreciate that you have chosen our facility to conduct your study and would like to remind you to please add a statement along these lines to acknowledge the Flow Cytometry Resource Facility in your publications and presentations:
"We thank Christina DeCoste (and/or John Grady) and the Molecular Biology Flow Cytometry Resource Facility which is partially supported by the Cancer Institute of New Jersey Cancer Center Support Grant (P30CA072720)."
Christina J. DeCoste, Director
(After hours sorting appointments are available upon request)
Unassisted/Self-Use for approved, trained users:
10am - 6pm
Princeton University, Dept. of Molecular Biology
|Princeton, NJ 08544|
|Christina J. DeCoste||