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NMR Core Facility

 

            Important Notice: COVID-19 and New SOP

All users must read and understand the new standard operating procedure

The NMR Facility has only one Entry (on the TA Auditorium side) and one Exit (next to the A400) for now.


There are four sectors -- (i) A1/NB3/cluster computers, (ii) A2/A3, (iii) A400, and (iv) A500, respectively -- for strict social distancing, one user at a time!


You MUST follow the path shown by the signs for in and out movement!


Signing up through our webpage using the WASE calendar for 10 minute blocks of time is required, multiple consecutive blocks are acceptable if needed, one empty block must be kept between users.  No exceptions!


Regular PPE is required, no outside gloves from the lab are welcome!


You must use a new plastic sheet every time to cover the keyboard and mouse, discard it after finished!  For touching spinners and tubes use kimwipes.


Personal communication should be kept at minimum, ask for assistance through the glass!


Phone numbers for quick access are 609-933-3528 (cell for István, for text, too) and 609-258-1633 (Ken's office). 

 

Welcome to the Princeton NMR Core Facility!

The Department of Chemistry houses a world-class nuclear magnetic resonance (NMR) facility with outstanding instrumental power and experimental capabilities.

  

Facility Use

To request training, consultation, and/or assisted services, please contact the core director.  The NMR Core Facility does not currently accept reservations via iLab, this page is for informational purposes only.

 

About the Facility

The NMR Core Facility is available 24/7 to fully trained and authorized users.

Staff assisted servicse are available Monday - Friday from 9am-5pm.

 

Instrumentation Available

  • A800 - Avance III 800MHz

    NEO console, 3 mm TCI cryoprobe, 24 holder SampleCase autosampler, 800MHz

    Highest sensitivity for proton detected experiments, triple resonance (1H/13C/15N) experiments, 2H detection and second for 13C detection. The 5 mm QCI-F cryoprobe is also available on demand.

  • A500 -Avance III console, RT BBFO (H/F, 31P-209Ag) probe, manual sample management, 500MHz

    1H, 19F, 31P, 13C, 11B and many other heteronuclei.

    Two HR-MAS probes (4 mm rotors) are also available, one 1H/13C/15N and another one for 1H/13C/14N.

  •  A1 - NEO console, TCI cryprobe (H/F,C,N), 60 holder SampleCase autosampler, 500MHz

    All proton detected experiments, triple resonance (1H/13C/15N) experiments, 2H detection and capable for 13C detection.

  • A2 - NEO console, tuneable cryoprobe (H/31P-15N), 60 holder SampleCase autosampler, 500MHz

    Direct detection of all heteronuclei with high sensitivity, quite good for 1H, too, 2H detection.

    A 5 mm RT high-power diffusion probe (up to 1,700 G/cm gradient strength, water-cooled, H/F,31P-15N) alternating periodically on demand.

  • A3 - NEO console, C-H DUAL cryoprobe, 60 holder SampleCase autosampler, 500MHz

    Unique for 13C detection (unparallel sensitivity), and very good for 1H as well.

  • NB300 - NanoBay console, RT BBFO probe, (H-F, double resonance available, 31P-209Ag), 60 seat SampleCase autosampler, 300MHz

    Workhorse machine, also for very low temperature (-100oC) experiments.

  • A400 - NanoBay console, Prodigy BBO nitrogen-cooled cryoprobe, 60 holder SampleXpress autosampler (takes any tall tube) 400MHz

    Competitive for all heteronuclei from -40oC to +150oC

  • ssNMR/DNP instrument

    This is a latest generation Bruker 400 MHz WB system with two probes for conventional ssNMR applications (using 3.2 mm and 1.3 mm rotors, respectively) and NEO console. One probe (3.2 mm) is a two-channel tunable multinuclear probe, the 1.3 mm probe is triple resonance with several nuclei available using inserts – the collection of nuclei is expandable. The maximum speed for the 3.2 mm rotor is 25 kHz, the 1.3 mm rotor was tested at 65+ kHz, which allows proton-detected sophisticated experiments.

    Each probe has a wide range of temperature control.

    In addition, the instrument has a DNP (Dynamic Nuclear Polarization) accessory with klystron-based MW source and two probes also (with 3,2 mm and 1.3 mm rotors, respectively). The DNP enhancement may exceed 100+-fold sensitivity increase, allowing drastic shortening of the experiment time or accessing low sensitivity nuclei or very small quantity samples. The default operation is at 100K using nitrogen cooling, supported by the latest, third generation temperature controller unit. The probes are multinuclear with inserts and at RT they can be used as conventional ssNMR probes as well. 

All instruments are controlled by TopSpin 4.3/3.5pl7 software and can run a large variety of 1D and 2D/nD correlation experiments, water suppression is routine. The liquid state instruments are equipped with ICONNMR automation interface.

The default processing software MestreNova, NMRPipe recommended for nD experiments.

 

Contact Information

István Pelczer, Director 

609-258-2342 

e-mail: ipelczer@princeton.edu

 

Kenith Conover, NMR Specialist
 
609-258-1633

e-mail: kenithc@princeton.edu

 

Location and Hours of Operation

Hours

Assisted Services    9am-5pm, Monday-Friday

Unassisted/Self-Use for approved and trained users   24/7

 

Location

Frick Chemistry Laboratory, B06

   
 

 

 

Contacts

Name Role Phone Email Location
Istvan Pelczer
Director
 
609-258-2342
 
ipelczer@princeton.edu
 
Frick, B09
 
Kenith Conover
NMR Specialist
 
609-258-1633
 
kenithc@princeton.edu
 
Frick, B10