Small Instruments Core Facility

COVID-19 and new SOP

Standard Operating Procedure - Small Instruments Core (Room B-08) 

1. This room has a maximum occupancy capacity of one person. Therefore, only one researcher can occupy the Chemical Instrumentation Room, even though there are eight different instruments available for use.
2. Reservations can be made using the Agilent’s I-Labs reservation system. As you login, using your account, there is a list of eight instruments available for signup.
3. Make your reservation on the calendar for the “Agilent CARY 5000 UV-VIS” instrument only! DO not signup on the calendars for any of the other instruments. The other seven instruments will be blocked and made unavailable for signup. The logic, is to arbitrarily just use one of the calendars for reservations, this will limit the signup to a single user in reserving the room. The researchers can make a reservation on this single calendar and reserve the room but can arrive and use any of the eight available instruments.
4. Make a reservation on the calendar for the “CARY 5000 UV-VIS Instrument, and leave a 15-30 min gap between reservations to prevent overlap between users. This buffer time, also allows the previous user to cleanup and wipe down all surfaces he/she has touched during their instrument usage. Arrive promptly in the lab with your prepared samples, if possible, at the time you reserved. Continue as before with signing of the logbooks for the specific instrument that you used.
5. If you arrive and see there is a user occupying any of the eight instruments, at the time you have reserved, simply ask or send an email John Eng to help resolve reservation issues.
6. When entering the Chemical Instrument facility (it is recommended you have) have on gloves and a mask.
7. After using the instrument, wipe down all touched surfaces with 80% ethanol in the spray bottles provided at the door. Prepare your samples with the proper dilutions before arriving, if possible and keep sample preparation and handling in the facility to a minimum

================End of SOP================

Welcome to the Princeton Small Instruments Core Facility!

The Small Instruments Core Facility houses a wide range of instrumentation dedicated to support both vibrational and electronic spectroscopy.

Facility Use

To request training, consultation, and/or assisted services, please contact the core director.  The Small Instruments Core Facility does not currently accept reservations via iLab, this page is for informational purposes only.

About the Facility

The Small Instruments Core Facility is available 24/7 to fully trained and authorized users. 

Staff assisted servicse are available Monday - Friday from 8:30am-5pm.

Instrumentation Available

 Ground State Electronic Spectra

• 8453 UV-VIS (Diode Array)

o   Routine instrument with a single 1 cm sample holder

• CARY 5000 UV-VIS

o   Equipped with a QUANTUM Northwest Dual Temperature-Controlled Cuvette Holder for Protein (or RNA/DNA) Stability; Melting; and aggregation measurements. Additionally an Integrating Sphere accessory is available for highly Turbid and/or high OD samples for this instrument

• CARY 60 UV-VIS

o   Standard instrument for absorption and kinetics measurements

• CARY Eclipse Fluorescence Spectrometer

o   Routine fluorescence measurement with a 1 cm sample holder

Ground State Vibrational Spectra

• IN10 Microscope

o   Manual sample stage IR microscope

• IS50 FT-IR

o   Research grade FT-IR with ATR and Transmission sample holder

• Nicolet 6700 FTIR

o   Research grade FT-IR with ATR sample accessory

• Nicolet DXR Raman

o   Raman instrument with 180 backscatter sample collection

Optical Rotation

• JASCO 2000 Polarimeter

o   Simple user-friendly standard optical rotation measurement instrument

Contact Information

John Eng, Director, Mass Spectroscopy and Small Instrument Core Facilities

609-258-3928

eng@princeton.edu

Location and Hours of Operation

Hours

Assisted Services    8:30am-5pm, Monday-Friday

Unassisted/Self-Use for approved and trained users   24/7

Location

Frick Chemistry Laboratory, B08

Contacts