Biophysics Core Facility

 

            Important Notice: COVID-19 and New SOP

All users must read and understand the new standard operating procedure  

 

                 New Standard Operating Procedure - Biophysics Core Facility

 

  • Please contact the core facility manager, Venu Vandavasi, if you have any questions about the use of the facility.
  • The layout of biophysics core facility allows a maximum of THREE users occupying three different areas in the core at any point of time.
  • There are three groups of instruments and only one user can access one instrument in a group at any point of time to comply with social distancing.
  • Ther should be atleast 30 minutes of buffer time between two consecutive users.
  • You must check the status of instruments in a particular group on iLAB before you sign up for reservation.

 Reservations

  • All users should use iLAB to check availability and to make instrument reservations.
  • Instruments are grouped as below:
    • Group A - AUC, CD, DLS and Stopped Flow
    • Group B - ITC, SPR and DSC
    • Group C - Fluorimeter, Plate reader, MST and OCTET
  • Only one instrument in a group should be booked/used at any given time.
  • Make sure you leave at least 30 minutes of buffer time if you notice any usage in the group of instruments you intend to work and sign up accordingly. 

 Proper use of the facility

  • All users must use tiger card to access the facility.
  • All users are required to use face masks and gloves inside the facility.
  • All users are highly recommended to wear a lab coat.
  • Please do not bring others for any reason such as assistance, training or teaching.
  • Confine yourself to your work area and stay as far as possible from other users.
  • Please make sure to clear your work area before you leave the core facility.

 Hygiene

  • Please use the paper towels/napkins to open the doors.
  • Soap and/or sanitizer is available near the sink area.
  • Please clean the work area before and after your experiment.
  • 70% Ethanol should be used to clean work bench.
  • Wet wipes must be used to clean the instruments only if necessary.
  • The saran wrap on the key boards will be changed by the core personnel.

 ===========================End of new SOP================================

 

Welcome to the Princeton Biophysics Core Facility!

The Biophysics Core Facility houses a wide range of instrumentation dedicated to support biophysical characterization of proteins, DNA and several other biological macromolecules and their interactions. The newly established core facility is a joint venture of the Department of Chemistry and the Department of Molecular Biology.

Facility Use

To request training, consultation, and/or assisted services, please register with iLab.

Getting Started with iLab:

  • Princeton Clients:  If you already have an account, log in (upper right corner) and go to the Schedule Equipment tab.  If you are a new user, log in (upper right corner) using a valid Princeton ID.
  • Non-Princeton Clients:  Please email vgv@princeton.edu directly with any questions about use of this facility. 
  • Visit the iLab Help Site or Customer Help Manual for further guidance using iLab's Core Facility Services.

 

About the Facility

The Biophysics Core Facility is available 24/7 to fully trained and authorized users.

Staff assisted services are available Monday - Friday from 8am-5pm.

 

Instrumentation Available

    • Analytical Ultracentrifuge (AUC)
      Instrument: Optima AUC from Beckman Coulter Life Sciences with absorbance and interference detection modules, two-rotors, An-60 Ti and An-50 Ti are available.
      Applications: Determination of molecular mass, aggregation, oligomeric states and stoichiometry, conformation and shape of proteins in solution.

 

    • Biolayer interferometry (OCTET)
      Instrument: Forte bio OCTET Red 96e with temperature control
      Applications: Analysis of biomolecular kinetics (rates of association and dissociation), binding affinities and quantification of macromolecules.

 

    • Circular Dichroism Spectrometer (CD)
      Instrument: Chirascan CD spectrometer from Applied Photophysics.
      Applications: Analysis of secondary structure of proteins and chiral molecules, binding and melting studies.

 

    • Dynamic Light Scattering (DLS)
      Instrument: DynaPro Nanostar from Wyatt technology
      Applications: Analysis of homogeneity of biological samples (protein solutions etc), determination of hydrodynamic radius, molecular masses and oligomeric states.

 

    • Fluorimeter
      Instrument: Fluorolog from Horiba with temperature control and fluorescence anisotropy modules
      Applications: Measurement of fluorescence and fluorescence anisotropy, binding and melting studies.

 

    • Isothermal Titration Calorimetry (ITC)
      Instrument: MicroCal PEAQ-ITC
      Applications: Measurement of binding affinities, enthalpy and entropy changes.

 

    • Microscale Thermophoresis (MST)
      Instrument: Monolith NT.115 (Blue/Red) from NanoTemper
      Applications: Measurement of binding affinities.

 

    • Multimode Plate Reader
      Instrument: Spectramax iD5 from Molecular Devices
      Applications: Measurement of Absorbance, fluorescence, fluorescence polarization, luminescence and TRF in 96 and 384 well formats.

 

    • Stopped Flow
      Instrument: SX20 Stopped-Flow spectrometer from Applied Photophysics. A Single mixing chamber, a monochromator for excitation and different filters for emission are available.
      Applications: Biomolecular Kinetics.

 

    • Surface Plasmon Resonance (SPR)
      Instrument: Biacore 3000 from GE Healthcare
      Applications: Analysis of biomolecular kinetics (rates of association and dissociation) and binding affinities.

 

Contact Information

Venu Vandavasi, Biophysics Facility Manager

phone: 609-258-0236

e-mail: vgv@princeton.edu

 

Location and Hours of Operation

Hours

Assisted Services    8am-5pm, Monday-Friday

Unassisted/Self-Use for approved and trained users     24/7

 

Location

Frick Chemistry Laboratory, B16

 

   
   

Contacts

Name Role Phone Email Location
Venu Vandavasi
Biophysics Facility Manager
 
609-258-0236
 
vgv@princeton.edu
 
Frick Chemistry Lab, B22