ALERT

As of December 31st, 2019, the TLS 1.0 protocol will no longer be supported by Agilent, including older browsers (e.g. IE 10, Safari 6, Firefox 26 and older) and users will not have access to the iLab sites. Please click here for more information.

Biophysics Core Facility

Welcome to the Princeton Biophysics Core Facility!

The Biophysics Core Facility houses a wide range of instrumentation dedicated to support biophysical characterization of proteins, DNA and several other biological macromolecules and their interactions. The newly established core facility is a joint venture of the Department of Chemistry and the Department of Molecular Biology.

Facility Use

To request training, consultation, and/or assisted services, please register with iLab.

Getting Started with iLab:

  • Princeton Clients:  If you already have an account, log in (upper right corner) and go to the Schedule Equipment tab.  If you are a new user, log in (upper right corner) using a valid Princeton ID.
  • Non-Princeton Clients:  Please email vgv@princeton.edu directly with any questions about use of this facility. 
  • Visit the iLab Help Site or Customer Help Manual for further guidance using iLab's Core Facility Services.

 

About the Facility

The Biophysics Core Facility is available 24/7 to fully trained and authorized users.

Staff assisted services are available Monday - Friday from 8am-5pm.

 

Instrumentation Available

    • Analytical Ultracentrifuge (AUC)
      Instrument: Optima AUC from Beckman Coulter Life Sciences with absorbance and interference detection modules, two-rotors, An-60 Ti and An-50 Ti are available.
      Applications: Determination of molecular mass, aggregation, oligomeric states and stoichiometry, conformation and shape of proteins in solution.

 

    • Biolayer interferometry (OCTET)
      Instrument: Forte bio OCTET Red 96e with temperature control
      Applications: Analysis of biomolecular kinetics (rates of association and dissociation), binding affinities and quantification of macromolecules.

 

    • Circular Dichroism Spectrometer (CD)
      Instrument: Chirascan CD spectrometer from Applied Photophysics.
      Applications: Analysis of secondary structure of proteins and chiral molecules, binding and melting studies.

 

    • Dynamic Light Scattering (DLS)
      Instrument: DynaPro Nanostar from Wyatt technology
      Applications: Analysis of homogeneity of biological samples (protein solutions etc), determination of hydrodynamic radius, molecular masses and oligomeric states.

 

    • Fluorimeter
      Instrument: Fluorolog from Horiba with temperature control and fluorescence anisotropy modules
      Applications: Measurement of fluorescence and fluorescence anisotropy, binding and melting studies.

 

    • Isothermal Titration Calorimetry (ITC)
      Instrument: MicroCal PEAQ-ITC
      Applications: Measurement of binding affinities, enthalpy and entropy changes.

 

    • Microscale Thermophoresis (MST)
      Instrument: Monolith NT.115 (Blue/Red) from NanoTemper
      Applications: Measurement of binding affinities.

 

    • Multimode Plate Reader
      Instrument: Spectramax iD5 from Molecular Devices
      Applications: Measurement of Absorbance, fluorescence, fluorescence polarization, luminescence and TRF in 96 and 384 well formats.

 

    • Stopped Flow
      Instrument: SX20 Stopped-Flow spectrometer from Applied Photophysics. A Single mixing chamber, a monochromator for excitation and different filters for emission are available.
      Applications: Biomolecular Kinetics.

 

    • Surface Plasmon Resonance (SPR)
      Instrument: Biacore 3000 from GE Healthcare
      Applications: Analysis of biomolecular kinetics (rates of association and dissociation) and binding affinities.

 

Contact Information

Venu Vandavasi, Biophysics Facility Manager

phone: 609-258-0236

e-mail: vgv@princeton.edu

 

Location and Hours of Operation

Hours

Assisted Services    8am-5pm, Monday-Friday

Unassisted/Self-Use for approved and trained users     24/7

 

Location

Frick Chemistry Laboratory, B16

 

   
   

Contacts

Name Role Phone Email Location
Venu Vandavasi
Biophysics Facility Manager
 
609-258-0236
 
vgv@princeton.edu
 
Frick Chemistry Lab, B22