All Biophysics Core Users:
Welcome to the Princeton Biophysics Core Facility!
The Biophysics Core Facility houses a wide range of instrumentation dedicated to support biophysical characterization of proteins, DNA and several other biological macromolecules and their interactions. The newly established core facility is a joint venture of the Department of Chemistry and the Department of Molecular Biology.
Facility Use
To request training, consultation, and/or assisted services, please register with iLab.
Getting Started with iLab:
- Princeton Clients: If you already have an account, log in (upper right corner) and go to the Schedule Equipment tab. If you are a new user, log in (upper right corner) using a valid Princeton ID.
- Non-Princeton Clients: Please email vgv@princeton.edu directly with any questions about use of this facility.
- Visit the iLab Help Site or Customer Help Manual for further guidance using iLab's Core Facility Services.
About the Facility
The Biophysics Core Facility is available 24/7 to fully trained and authorized users.
Staff assisted services are available Monday - Friday from 8am-5pm.
Instrumentation Available
- Analytical Ultracentrifuge (AUC)
Instrument: Optima AUC from Beckman Coulter Life Sciences with absorbance and interference detection modules, two-rotors, An-60 Ti and An-50 Ti are available.
Applications: Determination of molecular mass, aggregation, oligomeric states and stoichiometry, conformation and shape of proteins in solution.
- Biolayer interferometry (OCTET)
Instrument: Forte bio OCTET Red 96e with temperature control
Applications: Analysis of biomolecular kinetics (rates of association and dissociation), binding affinities and quantification of macromolecules.
- Circular Dichroism Spectrometer (CD)
Instrument: Chirascan CD spectrometer from Applied Photophysics.
Applications: Analysis of secondary structure of proteins and chiral molecules, binding and melting studies.
- Dynamic Light Scattering (DLS)
Instrument: DynaPro Nanostar from Wyatt technology
Applications: Analysis of homogeneity of biological samples (protein solutions etc), determination of hydrodynamic radius, molecular masses and oligomeric states.
- Fluorimeter
Instrument: Fluorolog from Horiba with temperature control and fluorescence anisotropy modules
Applications: Measurement of fluorescence and fluorescence anisotropy, binding and melting studies.
- Isothermal Titration Calorimetry (ITC)
Instrument: MicroCal PEAQ-ITC
Applications: Measurement of binding affinities, enthalpy and entropy changes.
- Microscale Thermophoresis (MST)
Instrument: Monolith NT.115 (Blue/Red) from NanoTemper
Applications: Measurement of binding affinities.
- Multimode Plate Reader
Instrument: Spectramax iD5 from Molecular Devices
Applications: Measurement of Absorbance, fluorescence, fluorescence polarization, luminescence and TRF in 96 and 384 well formats.
- Stopped Flow
Instrument: SX20 Stopped-Flow spectrometer from Applied Photophysics. A Single mixing chamber, a monochromator for excitation and different filters for emission are available.
Applications: Biomolecular Kinetics.
Contact Information
Venu Vandavasi, Ph.D.
Director & Consultant, Biophysics Research Core Facility
Office B-12
Princeton University
Phone (609) 258-0236
https://chemistry.princeton.edu/research-facilities/biophysics-core-facility
e-mail: vgv@princeton.edu
Location and Hours of Operation
Hours
Assisted Services 8am-5pm, Monday-Friday
Unassisted/Self-Use for approved and trained users 24/7
Location
Frick Chemistry Laboratory, B25 and B06b
Welcome!
