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Mass Spectrometry Facility

COVID-19 and new SOP

Standard Operating Procedure - Mass Spec Core Facility (Room B-14) 

  • The Room has a Maximum Occupancy Capacity of Four Persons. Researchers should work and stand within the Orange Working Zones outline in the diagram below for proper distancing. The five instruments available for reservations are grouped in different Zones.(See posted diagram of working areas for this room at the entrance of B-14, to maintain proper distancing of researchers)
  • Reservations can be made using the Agilent’s I-Labs reservation system. As you login, using your account, there is a list of ten instruments available for signup. Only the following five instruments listed below are available for signup at this time. The other five are future MS installations and are blocked as unavailable and you should not sign-up on these instruments. After reserving one of the available instruments, on the calendars, you should leave a 15-30 min gap between reservations, to prevent overlap between users. This time also allows the previous user to cleanup and wipe down all surfaces that have been touched during their instrument usage.
  • Arrive promptly in the lab with your samples, at your reserved time. Continue as before with signing of the logbooks with the number of samples you run on the Agilent 6230 and EPR; and also tape into the logbook(s) , as before, the worklist printout for all your GC-MS runs.

The five available instrument now are: (the other five are coming online shortly)

Agilent 7200 GC/QTOF

Agilent 5977 GC-MSD with 8890 GC

Agilent 5975 GC/MSD with 7890 GC

Agilent 6230 High Resolution ESI-TOF

Bruker EMXplus Electron Paramagnetic Resonance instrument.

  •  If you see that there are already four persons working in the lab or occupying the specific instrument you have reserved, upon your timely arrival, simply find or send an email to John Eng to help resolve reservation issues.
  • When entering the MS facility (it is recommended you have) have on gloves and a mask.
  • After using the instrument, wipe down all touched surfaces with 80% ethanol in the spray bottles provided at the door. If possible prepare your samples with the proper dilutions before arriving, and keep sample preparation and handling in the MS facility to a minimum.

================End of SOP================


Welcome to the Princeton Mass Spectrometry Facility!

The Mass Spectrometry Facility in the Department of Chemistry offers first-rate chemical analysis. Researchers use the facility for a variety of needs, from investigating the structure of key intermediates to obtaining publication quality spectra.

Facility Use

**NOTE: All customers MUST be trained in order to operate the equipment in this facility. If you have not received training, please schedule an appointment with John Eng ( or Venu Vandavasi (

To request access, training, consultation, and/or assisted services, please register with iLab.

Getting Started with iLab:

  • Princeton Clients:  If you already have an account, log in (upper right corner) and go to the Schedule Equipment tab.  If you are a new user, log in (upper right corner) using a valid Princeton ID.
  • Non-Princeton Clients:  Please email or directly with any questions about use of this facility. 
  • Visit the iLab Help Site or Customer Help Manual for further guidance using iLab's Core Facility Services.

About the Facility

The Mass Spectrometry Facility is available 24/7 to fully trained and authorized users.

Staff assisted services are available Monday - Friday from 8:30 am - 5:00 pm.


Frick Chemistry Laboratory, B11

Instrumentation Available

  • Mass Spectrometry
    • Agilent 7200 GC/QTOF
      • High Resolution GC-MS System for accurate-mass identification of organic compounds with EI+ ionization.
    • Agilent 7250 GC/QTOF
      • High Resolution GC-MS System for accurate-mass identification of organic compounds with EI+ ionization.
    • Agilent 5977 GC-MSD 8890GC
      • Unit Mass resolution GC-MS System for identification of organic compounds with EI+ ionization.
    • Agilent 5975 GC/MSD 7890GC
      • Unit Mass resolution GC-MS System for identification of organic compounds with EI+ ionization.
    • Agilent 6495C LC/QQQ
      • LC-QQQ instrument for metabolomics. Optimized for high sensitivity with a large dynamic range for peptide quantitation.
    • Agilent 6546 LC/QTOF
      • High resolution ESI-Q-TOF instrument. Optimized with high acquisition speed and wide dynamic range for proteomics and metabolomics.
    • Agilent 6545XT LC/QTOF
      • High resolution ESI-Q-TOF instrument. Optimized for peptide mapping and finding PTMs; also configurable for small molecule screening with good isotope fidelity and accurate mass measurements.
    • Agilent 6230 LC/TOF #1
      • Walk-up High-Resolution ESI-TOF Instrument for small molecule accurate mass measurements and molecular formula confirmation.
    • Agilent 6230 LC/TOF #2
      • Walk-up High-Resolution ESI-TOF Instrument, optimize for biomolecules in the negative ionization mode. (For the analysis of Oligonucleotides, DNA and associated modifications)
    • Bruker AUTOFLEX MAX MS
      • Matrix assisted laser desorption ionization, MALDI MS
    • Bruker TIMS TOF Flex 2 MS
      • Nano Elute 2 LC for Proteomics and MALDI for top-down Proteomics
  • Electron Paramagnetic Resonance
    • Bruker EMXplus
      • Several resonators are available for a wide range of applications. An Oxford liquid helium cryostat is installed on this instrument for low temperature sample handling down to 10K. 


Name Role Phone Email Location
John Eng
B11 Frick Chemistry Lab 
Venu Vandavasi
Analytical Spectroscopist
B25 Frick Chemistry Lab